KMID : 0368420150580010068
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Journal of Plant Biology 2015 Volume.58 No. 1 p.68 ~ p.73
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OsCML4 improves drought tolerance through scavenging of reactive oxygen species in rice
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Yin Xu Ming
Huang Li Fanng Zhang Xin Wang Man Ling Xu Guo Yun Xia Xin Jie
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Abstract
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Calmodulin-like (CML) genes function in regulating plant responses to different abiotic stresses, such as high salt and drought. Using japonica type rice seedlings (¡®Nipponbare¡¯), we induced the expression of OsCML4 (Accession No. NM_001057768), which encodes a rice CML protein. Under drought conditions, transgenic plants over-expressing that gene exhibited obviously improved growth performance and higher survival rates than the wild type (WT). Activity by reactive oxygen species (ROS)- scavenging enzymes superoxide dismutase (SOD) and catalase (CAT), as well as proline concentrations, were significantly increased in transgenic plants. Expression by ROS scavenging-related genes APXI and Cat-B and the stress-related gene OsP5CS1 was also enhanced in the transgenics under drought conditions. However, the sensitivity to abscisic acid (ABA) was similar between over-expressing and WT plants. These results suggest that OsCML4 confers drought tolerance partially through ROS-scavenging and also by inducing other stress-related genes in an ABAindependent manner.
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KEYWORD
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Calmodulin-like gene, Drought stress, Reactive oxygen species, Rice (Oryza sativa L.)
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